Server cannot access application director

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can anyone help me with  security settings in windows 2003 ?
Server cannot access application directory 'E:\Documents and Settings\Administrator\Desktop\samples\'. The directory does not exist or is not accessible because of security settings. 
[HttpException (0x80004005): Server cannot access application directory 'E:\Documents and Settings\Administrator\Desktop\samples\'. The directory does not exist or is not accessible because of security settings.]